ABSTRACT
Anti-diabetic and hypolipidemic effects of seed of Gossypium herbaceum L [GH] and its aqueous and ethanol extracts were investigated in alloxan-induced diabetic rabbits. Normal, Alloxan-induced diabetic and treated groups of rabbit were examined for their serum glucose, triglyceride, cholesterol, creatinine and urea levels. Water/food intake and toxic effect of test substances were also observed in treated rabbits. Effect of test agents on architecture of pancreatic beta-cells was evaluated histopathologically in rabbits. GH powder, its aqueous [GHA] and ethanol [GHE] extract significantly [P<0.05] reduced normoglycemia, serum cholesterol, triglyceride and urea in a dose dependent order [200-300 mg/kg of body weight] in normal rabbits. GH and GHE ameliorated completely the Alloxan effect on serum levels of glucose, cholesterol, triglyceride, creatinine and urea in Alloxan-induced diabetic rabbits. GHA and Glimepiride [a reference drug] partially blocked such effect of the Alloxan in treated rabbits. Further GH, GHA and GHE did not cause any change in food/water intakes and on liver, spleen, kidney, lung and heart in treated rabbits. Phytochemical study of GH and its extracts revealed the presence of flavonoids and phenolic compounds. Histopathological examination showed the protective effect of GH, GHA and GHE against Alloxan-induced destruction of beta-cells of pancreas in diabetic rabbits. Data indicated that GH and its aqueous and ethanol extracts have promising anti-diabetic and hypolipidemic effects. GH and GHE could be effective tool against the development, progression and complication of Diabetes mellitus
Subject(s)
Adult , Animals, Laboratory , Male , Phytotherapy , Gossypium , Plant Preparations/pharmacology , Hypoglycemic Agents , Alloxan , Rabbits , Hypolipidemic AgentsABSTRACT
Aim/Background: The present study was planned to evaluate the antidiabetic activity of ‘Folk recipe’ a combination of traditional medicinal plants in normoglycemic and alloxan-induced diabetes rabbits. The level of antioxidant activity was determined by DPPH in relation to the total phenolic contents. Methods: Antidiabetic activity of aqueous extract of Folk Recipe (AFR) in 100-300 mg/kg, b.w. doses was determined by estimating blood glucose and serum insulin levels before and 1, 2, 4, 8, 24, 48 and 72 hour post-treatment(s) intervals in treated rabbits. Total phenolic contents and DPPH-antioxidant activity of AFR were measured in vitro. Results: AFR showed a dose dependent antidiabetic activity; maximum effect was established with 300 mg/kg, b.w. dose. The extract exerted a high significant (P<0.001) hypoglycemic effect in normal and alloxan diabetic rabbits. Extract showed a significant (P<0.05) increase in insulin levels and protected completely against alloxan-induced histopathological changes in pancreatic beta-cells of diabetic rabbits. A high antioxidant activity of AFR (5-10 μg/mL) was observed in comparison with L-ascorbic acid (5-10 μg/mL). The doses used did not show any sign of acute toxicity or resulted in any behavioral change. Conclusion: From this study it may be concluded that the Folk recipe causes a reduction in blood glucose and increasing serum insulin levels may combat due to antioxidant activity by protecting beta-cells. Evaluation agreed with the potential use of Folk recipe as a traditional anti-diabetic tool.
ABSTRACT
The present study examines the prevalence of hepatitis [B andC] infection in population of the urban areas of Bahawalpur district. The study population comprised of 6815 peoples [3924 male, 2891 female] of different age group and professions. The subjects were selected randomly and had blood samples taken. Rapid immunochromatographic tests were conducted to detect hepatitis B and C infections. The prevalence of diseases was: hepatitis B, 9.59% and hepatitis C,4.41% indicating the spread of infections on rise in Pakistan. The results of the study illustrate the importanceof education of the society including health care individuals to observe the protective measures against hepatic viral infections and of maintaining the health care measures